Rnase A
⇓ Protocol
Rnase A or Ribonuclease A is an enzyme that specifically degrades single-stranded RNA by cleaving the phosphodiester bonds between nucleotides. It is commonly used in molecular biology for various applications, such as:
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Plasmid and genomic DNA preparation:
- To remove RNA contaminants.
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Removal of RNA from recombinant protein preparations:
- Ensuring the purity of the protein.
-
Ribonuclease ARNase protection assays:
- To map single-base mutations in DNA or RNA.
Ribonuclease A is active under a wide range of reaction conditions and is typically used at concentrations ranging from 1 to 100 µg/mL. It is important to handle Ribonuclease A with care, as it can degrade RNA in your samples if not properly controlled.
